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ISDN2014_0394: DENND5A regulates NGF‐induced neurite outgrowth in PC12 cells and dendrite patterning of primary hippocampal neurons
Author(s) -
Han Chanshuai,
Daubaras Mark,
McPherson Peter S.
Publication year - 2015
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2015.04.313
Subject(s) - neurite , microbiology and biotechnology , guanine nucleotide exchange factor , rab , hippocampal formation , gtpase , biology , nerve growth factor , small gtpase , neuroscience , regulator , nervous system , neurogenesis , embryonic stem cell , signal transduction , receptor , genetics , in vitro , gene
markers of modality discrimination have been found in the dorsal horn of the spinal cord. Using a candidate gene approach we focussed on the mouse transcription factor Lmx1b, which is expressed in developing dorsal horn neurons and essential for their normal development, but its role in nociception has not been characterised in postnatal animals. To assess the behavioral consequences of deleting Lmx1b in the spinal cord, we generated a conditional Lmx1b knockout mouse line using a spinal cord-specific Cre recombinase driver. Such mice show robustly lowered sensitivity to mechanical and inflammatory noxious stimulation but have normal thermal nociception. Since our preliminary analysis suggests that sensory axon projections are normal in Lmx1b conditional mutants, we are currently exploring the possibility that Lmx1b defines a population of dorsal horn neurons devoted to mechanical pain sensation, in line with the observation that human LMX1B mutations result in modality-specific nociception defects.