Premium
ISDN2014_0268: An alternative model to study the effects of early‐life stress on developmental neural plasticity in rodents
Author(s) -
Blaise J.H.
Publication year - 2015
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2015.04.219
Subject(s) - citation , psychology , computer science , cognitive science , library science
Objective: To compare the distribution and location of IP3R and RyR in ventricular and subventricular zones located in the wall of the lateral ventricle in the embryonic and adult rats. Methodology: We used Long Evans rats aged 16 days of embryonic (E16) and adult of approximately two months (P60). To evaluate these receptors in proliferative zones, it was used the immunofluorescence technique with specific antibodies for each of three IP3 receptor isoforms (IP3R1, IP3R2 and IP3R3) as well as for all three subtypes of ryanodine receptors (RyR1, RyR2 and RyR3). Results: The analysis revealed that tree isoforms of IP3 receptors and tree isoforms of ryanodine receptors are present in ventricular zone at E16, and their distribution is similar and present in all cells practically with cytoplasmic localization. In adult animals, the number of cell is less than E16, but the pattern of distribution these six receptors is similar with cytoplasmic characterization. Conclusion: The ventricular and subventricular zones show all isoforms of IP3R and RyR in both ages but with advantage in embryos, and the higher levels during embryonic development supporting the idea of the participation of calcium intracellular in the processes such as neurogenesis, cycle cell, and cell migration during the development of central nervous system.