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ISDN2014_0114: Decreased mTOR signaling via p70S6K/eIF4B is associated with loss of the excitatory postsynaptic marker PSD‐95 in autism
Author(s) -
Nicolini Chiara,
Fahnestock Margaret
Publication year - 2015
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2015.04.093
Subject(s) - excitatory postsynaptic potential , autism , psychology , editorial board , neuroscience , psychoanalysis , cognitive science , psychiatry , library science , computer science , inhibitory postsynaptic potential
Peripherial nerve injuries are very common (3–10%) in orthopaedic practice. Also treatment of them is continuous (Sunderland reports that satisfactory reinnervation of human muscle can occur after denervation of 12 months, sensory recovery occurs earlier), mostly patients are young, able-bodied. So the problem is actual for nowdays. There are three suturing techniques: neurorrhaphy, interfascicular nerve grafting (ING) and nerve grafting with tubes. We proposed a hypothesis that using ING with autological cells of stromal vascular fraction (SVF) can improve the results of treatment. In experiment on group of 22 Wistar rats (ING in sciatic nerve with gap 1 cm with transplantation SVF and group without it) we showed difference in appearing of sensitivity, electromyographic, histological, sciatic function index, laser Doppler investigations. We invented method of substituting peripheral nerve defect using cells of SVF on clinical trial. Method: abdominal liposuction, enzyme treatment of adipose tissue (which contains mesenchymal stem cells, endothelial precusors, M2 polarizing macrophages and T-lymphocytes), received cells of the SVF transplantated into peripheral, central nerve segments and in autonervegraft between them, the end of nerve paste covered with fibrin glue. Clinical example: Patient X. 25 years with diagnosis: «the consequences of the ulnar nerve damage in the upper third of the left shoulder». Defect ulnar nerve amounted to 5 cm. 25.08.11 operation: liposuction, receiving SVF. ING of ulnar nerve with the use of a calf nerve. Transplantation of SVF into peripheral, central nerve segments and in autonervegraft, covered with fibrin glue. 6 weeks after surgery the patient revealed painful sensibility in the autonomous area of innervation of the ulnar nerve, while the standard plastic appearance of pain sensitivity is celebrated on the timing is not less than 8–10 months after the operation. For this moment operated 10 patients, whom now examining on different terms.