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ISDN2014_0062: Absence of atypical E2f leads to ventriculomegaly
Author(s) -
DugalTessier D.,
Vandenbosch R.,
Khacho M.,
Weijts B.,
Park D.S.,
Leone G.,
Bruin A.,
Slack R.S.
Publication year - 2015
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2015.04.048
Subject(s) - library science , nobel laureate , medicine , political science , art , literature , poetry , computer science
biology, compelling evidence for a network of activity has been tributed to transcriptional molecules such as Oct4, Nanog and nestin, during neurogenesis, the choice between enhancement versus suppression of transcriptional modulation of some pluripotency and stem-associated genes would determine the balance between self-renewal neural stem cells (NSC) and immature neuronal phenotypes. Thus, by means of immunocytochemistry and RT-PCR methods, our study aimed to address the question whether and to what extent mRNA and protein profiles are expressed in human fetal neurospheres obtained from cortical and striatal brain regions. Morphological and biochemical analysis was carried out both in expansion (undifferentiated cells) and differentiation conditions monitored after 1 and 4 weeks in vitro culturing. Our results clearly demonstrated the sustained presence of opposite signals: strong downregulation of Oct4 and Nanog genes in cortical differentiating cells and significant up-regulation for nestin gene both in cortical and striatal differentiating cells. Notably, by immunostaining techniques, Oct4 and Nanog protein expression were detected not only in the nuclear compartment but also in the cytoplasmic domain followed by their rapid turnover (immediately after 1 week). In addition, in the course of the differentiation process, dissociated neurospheres displayed unexpected number of nestin positive cells accompanied by a constant level of staining intensity. In conclusion, the present study provides new insights into brain region related features in terms of Oct4, Nanog and nestin expression both at cellular and molecular level.