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Maternal omega‐3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth
Author(s) -
Rathod Richa S.,
Khaire Amrita A.,
Kale Anvita A.,
Joshi Sadhana R.
Publication year - 2015
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2015.04.006
Subject(s) - endocrinology , medicine , offspring , vitamin , fatty acid , docosahexaenoic acid , vitamin d and neurology , biology , vitamin e , pregnancy , polyunsaturated fatty acid , biochemistry , antioxidant , genetics
Vitamin B 12 and omega‐3 fatty acids are critical for normal brain development and function and their deficiencies during pregnancy could have adverse effects on cognitive performance in children. Our earlier studies indicate that both maternal vitamin B 12 and omega‐3 fatty acids influence brain development by regulating the levels of neurotrophins. Literature suggests that there exists a cross talk between neurotrophins like nerve growth factor (NGF) and angiogenic factors like vascular endothelial growth factor (VEGF). It remains to be established whether maternal nutrients like vitamin B 12 and omega‐3 fatty acids influence the levels of angiogenic markers like VEGF and NGF in the brain of the offspring. Therefore the present study examines the effect of maternal vitamin B 12 and omega‐3 fatty acids on protein and mRNA levels of VEGF, HIF‐1 alpha (hypoxia inducible factor alpha) and NGF in the pup brain at birth. Pregnant Wistar rats were divided into five dietary groups ( n = 8 each): control, vitamin B 12 deficient, vitamin B 12 deficient + omega‐3 fatty acid, vitamin B 12 supplemented, vitamin B 12 supplemented + omega‐3 fatty acid. At birth the pups were dissected to collect the brain tissue. Maternal vitamin B 12 deficiency showed lower ( p < 0.05) pup brain mRNA and protein levels ( p < 0.01) of VEGF, higher ( p < 0.01) HIF‐1 alpha protein levels, lower ( p < 0.05) NGF protein levels while NGF mRNA levels were not altered. Omega‐3 fatty acid supplementation to a vitamin B 12 deficient group normalized the VEGF mRNA levels, NGF protein levels and HIF‐1 alpha protein levels. Vitamin B 12 supplementation showed similar protein and mRNA levels of VEGF and NGF as well as HIF‐1 alpha protein levels as compared to control. Omega‐3 fatty acid supplementation to the vitamin B 12 supplemented group showed higher ( p < 0.01) protein and mRNA levels of NGF but the protein and mRNA levels of VEGF were comparable to control. In conclusion maternal vitamin B 12 and omega‐3 fatty acids both influence the levels and expression of neurotrophins and angiogenic factors in the offspring brain suggesting a possible benefit of combined maternal supplementation of these vital nutrients.