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Acute renal failure potentiates brain energy dysfunction elicited by methylmalonic acid
Author(s) -
Schuck Patrícia F.,
Januário Silvana B.,
Simon Kellen R.,
Scaini Giselli,
Mafioleti Renato L.,
Malgarin Fernanda,
Pettenuzzo Leticia F.,
Streck Emilio L.,
Ferreira Gustavo C.
Publication year - 2013
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2013.02.002
Subject(s) - creatine kinase , kidney , cerebral cortex , endocrinology , mitochondrial respiratory chain , medicine , gentamicin , renal cortex , pharmacology , chemistry , mitochondrion , biochemistry , antibiotics
The influence of acute renal failure induced by gentamicin administration on the effects of MMA on mitochondrial respiratory chain complexes, citrate synthase, succinate dehydrogenase and creatine kinase activities in cerebral cortex and kidney of young rats were investigated. Animals received one intraperitoneal injection of saline or gentamicin (70 mg/kg). One hour after, the animals received three consecutive subcutaneous injections of MMA (1.67 μmol/g) or saline (11 h interval between injections) and 60 min after the last injection the animals were killed. Acute MMA administration decreased creatine kinase activity in both tissues and increased complexes I–III activity in cerebral cortex. Creatine kinase activity was also inhibited by gentamicin administration. Simultaneous administration of MMA and gentamicin increased the activities of citrate synthase in cerebral cortex and kidney and complexes II–III in cerebral cortex. The other enzyme activities in cerebral cortex and kidney of animals receiving MMA plus gentamicin did not significantly differ from those observed in animals receiving only MMA. Our present data is line with the hypothesis that MMA acts as a toxin in brain and kidney of rats and suggest that renal injury potentiates the toxicity of MMA on the Krebs cycle and respiratory chain in brain and peripheral tissues.