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ISDN2012_0252: Genesis and development of dopaminergic interneurons in the adult brain
Author(s) -
Clarke Jessica,
Scheibe Mandy,
Goodison Tavia,
Cavanagh Brenton,
Meedeniya Adrian
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.10.051
Subject(s) - library science , research centre , otorhinolaryngology , medicine , gerontology , psychiatry , computer science
and GABAergic neurons to identify alterations in network dynamics produced by TBI in comparison to sham injured mice. We used GAD67 knock-in mouse that expressed GFP in all GABAergic neurons. In these mice, we recorded from three groups of cells: excitatory neurons (EGFP-negative and SR101-negative), GABAergic neurons (EGFP-positive and SR101-negative), and astrocytes (EGFP-negative and SR101-positive) with the goal to characterize cell-type specific TBI-induced dysfunction. We compared parameters like resting potential, voltage-dependent whole-cell currents, spontaneous activity (EPSPs) and action potential characteristics between cells in the proximity of the injury and in similar regions of slices from sham-operated animals. Combined measurements of membrane potential and calcium activity were performed to further elucidate the mechanisms underlying calcium transients. Our results suggest desynchronization of neocortical activity following TBI with an impaired inhibition underling hyperactivity/hypoactivity of excitatory neurons possibly due to disruption of feedback inhibition. Taken together the capacity to simultaneously image calcium dynamics in three different cell types with high spatial and temporal resolution along with whole-cell recordings in individual neurons promises to serve as a valuable tool for a comprehensive and quantitative analysis of molecular mechanisms in barrel cortex and thalamocortical circuit dysfunction underlying the pathophysiology of TBI.