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ISDN2012_0182: The indigenous gut microbiota modulates the blood–brain barrier in mice
Author(s) -
Anuar Farhana,
AlAsmakh Maha,
Miklos Toth,
Kwak YoungKeun,
Möllby Roland,
Bakocevic Nadja,
Guan Ng Lai,
Hibberd Martin L.,
Gulyas Balazs,
Halldin Christer,
Volpe Bruce T.,
Diamond Betty,
Pettersson Sven
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.10.010
Subject(s) - library science , agency (philosophy) , medicine , sociology , social science , computer science
Rashmi Mathur 1,∗, Suneel Kumar 1, Suman Jain 1, Sujata Mohanty 2, Jitendra Behari 3 1 Department of Physiology, All India Institute of Medical Sciences (AIIMS), New Delhi 110026, India 2 Stem Cell Facility, All India Institute of Medical Sciences (AIIMS), New Delhi 110026, India 3 School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110016, India Irreversible loss of motor, autonomic and altered sensory function including chronic pain distress patient and their families of spinal cord injured (SCI) patients. Sporadic reports exist in literature regarding the beneficial effects of bone marrow stromal cells (BMSCs) transplantation or exposure to magnetic fields (MF) in SCI while nadir for agonizing pain; albeit with limited success. We report effect of BMSCs and MF (17.96 T, 50 Hz, 2 h/d × 8 weeks) exposure on motor and pain in SCI adult male Wistar rats. Laminectomy was performed under ketamine anaesthesia in sham group while complete transection at T13 for SCI groups. SCI rats either received BMSCs on day 9 locally (SCI + BMSC group) or daily MF exposure from day 1 (SCI + MF group) or both (SCI + MF + BMSC group). Rat BMSCs were cultured and identify the presence of specific cell-surface antigens (CD44, CD90, CD45 and HLAII) using flow cytometry. PKH26 labelled BMSCs (∼2.5 × 105) were transplanted at the site of injury and rats were sacrificed at wk 8. BBB score for locomotion; threshold of tail flick (TF), simple vocalization (SV), vocalization after discharge (VAD), nociceptive flexion reflex (NFR), H-reflex were assessed pre and post SCI. BBB score, TTF, threshold of NFR and H-reflex recovered significantly (post-SCI wk 2–8, 4–8, 8, respectively) in SCI + MF, SCI + BMSCs SCI + BMSC + MF versus SCI group. BBB score were significantly higher (P < 0.05) post-SCI wk 5–7 in SCI + MF + BMSCs versus SCI + BMSCs/SCI + MF group. SV and VAD could not be elicited in any group post-SCI. BMSCs were observed in spinal cord around the injury site and were positive for neuronal, astrocytes and oligodendrocytes markers ( -III tubulin, GFAP and Olig4, respectively). Our results suggest the beneficial effect of BMSCs and MF exposure on locomotion, pain and electrophysiological parameters in thoracic complete spinal cord injured rats. Acknowledgements: We thanks to ICMR for their financial support.