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Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae ( Danio rerio )
Author(s) -
Capiotti Katiucia Marques,
Fazenda Lidiane,
Nazario Luiza Reali,
Menezes Fabiano Peres,
Kist Luiza Wilges,
Bogo Maurício Reis,
Da Silva Rosane Souza,
Wyse Angela Terezinha,
Bonan Carla Denise
Publication year - 2013
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.09.002
Subject(s) - danio , zebrafish , purinergic receptor , arginine , extracellular , biology , microbiology and biotechnology , biochemistry , medicine , endocrinology , amino acid , gene
Hyperargininemia is an inborn error of metabolism (IEM) characterized by tissue accumulation of arginine (Arg). Mental retardation and other neurological features are common symptoms in hyperargininemic patients. Considering purinergic signaling has a crucial role from the early stages of development and underlying mechanisms of this disease are poorly established, we investigated the effect of Arg administration on locomotor activity, morphological alterations, and extracellular nucleotide hydrolysis in larvae and adult zebrafish. We showed that 0.1 mM Arg was unable to promote changes in locomotor activity. In addition, 7‐day‐post‐fertilization (dpf) larvae treated with Arg demonstrated a decreased body size. Arg exposure (0.1 mM) promoted an increase in ATP, ADP, and AMP hydrolysis when compared to control group. These findings demonstrated that Arg might affect morphological parameters and ectonucleotidase activities in zebrafish larvae, suggesting that purinergic system is a target for neurotoxic effects induced by Arg.