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Osteopontin increases the proliferation of neural progenitor cells
Author(s) -
Kalluri Haviryaji S.G.,
Dempsey Robert J.
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.04.003
Subject(s) - osteopontin , progenitor cell , cyclin d1 , neural stem cell , cell growth , retinoblastoma protein , microbiology and biotechnology , cancer research , cell cycle , chemistry , downregulation and upregulation , cyclin e , biology , stem cell , cell , immunology , biochemistry , gene
We examined the role of osteopontin in the proliferation of neural progenitor cells in vitro . Osteopontin increased the proliferation of neural progenitor cells in the presence of FGF2 as measured by cell proliferation assay and bromodeoxy uridine incorporation studies. In addition, immunoblot analysis demonstrated an increase in the phosphorylation of retinoblastoma protein with a concurrent increase in the content of phospho‐Akt and cyclin D1. These results indicate that osteopontin can upregulate the content of phospho‐Akt, cyclin D1 and phospho‐Rb to subsequently enhance the proliferation of neural progenitor cells in the presence of FGF2.