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Neuroepithelium potential: Polarized currents produce extracellular voltage gradient
Author(s) -
Charles-Felix Calvo,
Jean-Léon Thomas,
Chunling Fan,
Mengqi Zhang,
Zhengyu,
Yang,
Lei Shang,
Jufang Huang
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.03.312
Subject(s) - neuroepithelial cell , citation , neuroscience , computer science , psychology , library science , operations research , biology , mathematics , microbiology and biotechnology , stem cell , neural stem cell
Neuroepithelial cells have polarized structure; the apical end of neuroepithelial cell faces the ventricle and the basal end touches he basement membrane. It is likely that the asymmetric structure enerates polarized ionic currents to cause a local change in the xtracellular potential. The present study has been aimed to reveal patial distribution of the extracellular potential that is standing ithin the neuroepithelium. To record the extracellular potential in neuroepithelium, the etinal neuroepithelium of chick embryo was used. An optic cup as dissected out from a chick embryo incubated for three days, nd placed on a recording chamber. Extracellular potential was ecorded with a microelectrode at the dorsal, temporal, nasal, cenral and ventral parts of the optic cup, just beneath the inner imiting membrane of the retinal neuroepithelium. A positive direct current (DC) potential was recorded there. The mplitude of the DC potential was largest at the dorsal part (8 mV),