Premium
Cell‐autonomous role of FGF receptors and Erk1/2 MAPK signaling in the regulation of myelination
Author(s) -
M. Furusho,
J. L. Dupree,
R. Bansal,
O. Riccio,
S. Murthy,
N. Hurni,
L. Hein,
A. G. Dayer
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.03.302
Subject(s) - mapk/erk pathway , citation , neuroscience , receptor , biology , signal transduction , library science , microbiology and biotechnology , computer science , genetics
markers Oct4 and SSEA4. An upregulation of it was observed in the outgrowths obtained from these neurosphere like structures on adherent matrix. In the presence of the midbrain cues the DPSCs showed an upregulation of the dopaminergic cell specific transcription factors Nurr1 and En1. Immunofluorescence analysis showed that the induced cells were positive for mature neuronal marker Map2ab and dopaminergic cell type markers like tyrosine hydroxylase (TH), PitX3 and Nurr1. Flow cytometry data depicted that ∼63% of the induced DPSCs were positive for TH. Thus our preliminary data suggests that the neural crest derivative DPSCs have a predisposition towards neuronal lineage. DPSCs were not only capable of forming neurosphere like clusters in non-adherent condition and NSC media but could also respond to midbrain cues and undergo transdifferentiation.