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Differential roles for ionic and metabolic glucosensing in a minimal model of VMH glucose‐excited neurons
Author(s) -
S. B. Dhanesh,
C. Subashini,
T. S. Divya,
Jackson James,
V. T. Abdul Rasheed
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.03.263
Subject(s) - citation , excited state , differential (mechanical device) , ionic bonding , computer science , library science , physics , atomic physics , thermodynamics , quantum mechanics , ion
similar to mesenchymal stem cells after one week in culture. These expanded cells were further characterized by FACS analysis with MSC specific markers such as CD 29 (88.5%) and CD 105 (87%) indicating their MSC lineage. These primary UCB-MSCs were exposed to neuronal differentiation condition and found that they could be very easily differentiated into neurons whereas the RCB 2080 hUCB-MSC cell line and other MSCs isolated from UCB had to be exposed to a series of growth factors to attain neurogenic potential. We also found a consistent expression of pluripotent markers such as Oct4, Nanog, Sox2, ABCG2 and neuro-ectodermal marker, Nestin in freshly isolated as well as proliferating MSCs indicating the presence of neural stem cell like properties within the population of MSCs. The neurogenic potential of different UCB-MSCs also varies from each other. Therefore, these results indicate that it is the small population of cells with inherent neurogenic potential that is differentiating into neurons in addition to trans-differentiation of MSCs. From these preliminary results we conclude that there exists a self-renewing neural stem cell like populations in umbilical cord blood which needs to be further characterized. Supported by funding from Dept. of Biotechnology & ICMR, Govt. of India.