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Dehydroepiandrosterone up regulates glial glutamate transporter expression, suppresses seizure and alter behavioral activity in experimental model of post‐traumatic epilepsy in rat brain
Author(s) -
Deepak,
Sharma
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.03.255
Subject(s) - epilepsy , excitatory amino acid transporter , neuroscience , glutamate receptor , psychology , epileptogenesis , rat model , dehydroepiandrosterone , transporter , medicine , endocrinology , biology , receptor , hormone , androgen , gene , biochemistry
ulatory pathways from yeast to mice suggest that insulin/IGF-1 signaling pathways may also regulate cell damage and longevity in humans. It has been reported that BDNF heterozygous mice develop an eating behavior disorder leading to obesity, and fat BDNF heterozygous mutant (FBH) mice are leptinand insulin-resistant. This indicates a possible cross-talk between the BDNF and IGF1 signaling pathways that may be involved in the control of various physiological processes, including food intake and metabolism, as well as ageing. The WNIN obese (WNIN/Ob) mutant rat model developed at the National Center for Laboratory Animal Sciences (NCLAS; NIN, Hyderabad) from the Wistar rat inbred colony (WNIN) exhibit hyperphagia, increased cholesterolemia, triglycerdemia, and leptinemia, with decreased lifespan. This is the first inbred rat mutant of its kind developed from an inbred laboratory stock and is currently the heaviest rat (∼=1.5 kg) of its kind in the world. In the present study we investigated the IGF1 and BDNF levels in plasma, cerebrospinal fluid, hypothalamus and cerebral cortex using BioPlex method which is a highly sensitive multiplex suspension array technique utilizing the principle of capture sandwich immunoassay to measure protein levels in tissue extracts and biological fluids. The plasma IGF1 levels were found to be increased albeit not significantly in WNIN/Ob rats whereas the BDNF levels showed significant decrease in the plasma and hypothalamus. The BDNF levels were comparatively lower in CSF but interestingly there were no changes in the cerebral cortex. Therefore, it is possible that the titer values of these neurotrophic factors in the brain and periphery and also their altered signaling may play a role in decreasing the lifespan of WNIN/Ob rats. Authors acknowledge the research grants and SRF to JKS from ICMR and financial support to JKS from ISN and IBRO.