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Influence of postnatal treatment with fluoxetine on gene expression, neurogenesis and mood related behavior
Author(s) -
Ankita Pandey,
Aruna Shukla,
A. B. Pant,
Devendra Parmar,
Sanjay,
Yadav,
Lynette D’Souza,
Sap. Shetty,
A. Vidita,
Vaidya,
Saborni Roy Das,
Tapas C. Nag,
Rashmi Mathur,
Suman Jain,
Anurag Sharma
Publication year - 2012
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2012.03.234
Subject(s) - neurogenesis , fluoxetine , mood , psychology , citation , mania , neuroscience , psychiatry , library science , biology , genetics , bipolar disorder , computer science , serotonin , receptor
MicroRNAs (miRNAs) are small (∼22 nucleotide) non-protein coding endogenous RNA molecules that post-transcriptionally regulate expression of protein coding genes. Recent studies have indicated that miRNAs are involved in specific neuronal functions and are rapidly emerging as key regulators of neuronal development and function. Nerve growth factor (NGF) differentiated PC12 cells (rat pheochromocytoma cells) are gaining significance as an in vitro model to study neuronal development and differentiation. Our, knockdown studies of dicer (protein responsible for formation of mature and functional miRNAs) have showed that expression of mature miRNAs is essential for proper differentiation of PC12 cells. Silencing of dicer has found to alter the pattern and quantity of neurite formation. When dicer silenced cells were exposed with NGF they have shown formation of more neurite but with reduced length in comparison to the cells transfected with non targeting control siRNA. Using Taqman Low Density Array (TLDA), we had carried out global expression profiling of miRNAs in undifferentiated and NGF differentiated PC12 cells. After profiling of 728 miRNAs, we had identified miRNAs which were up-regulated (miR-345-3p; miR-302b, miR-34c, miR-7b, miR-222) or down-regulated (miR-423, miR-489, miR-145, miR-383, miR451) during NGF induced differentiated of PC12 cells. Further, using MetaCore software suite we have identified neurogenesis, cytoskeleton rearrangement as major processes targeted by miRNAs regulated during differentiation. In summary, we had shown that NGF induced differentiation of PC12 cells involves regulation of set of miRNAs and expression of dicer is necessary for proper differentiation of PC12 cells.