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Spatial and temporal expression of vasodilator‐stimulated phosphoprotein (VASP) in fetal and adult human cerebral cortex
Author(s) -
Yaba Aylin,
Kayisli Umit,
Tanriover Gamze,
Demir Necdet
Publication year - 2011
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2010.12.004
Subject(s) - phosphoprotein , neocortex , cerebral cortex , biology , human brain , immunostaining , microbiology and biotechnology , angiogenesis , western blot , cortical dysplasia , neuroscience , pathology , immunohistochemistry , medicine , immunology , phosphorylation , cancer research , genetics , gene , epilepsy
Vasodilator‐stimulated phosphoprotein (VASP) is a member of the Ena/VASP protein family and was for the first time identified in human platelets. VASP plays a role for cell adhesion and cell migration because of E/DFPPPPXD/E amino acid sequence in its structure which gives reaction with focal adhesion proteins. In this study we suggest that VASP expression may have important role for neural cell migration, differentiation, axonal growth and angiogenesis during prenatal cerebral cortical development. Our aim is to detect VASP expression by means of immunohistochemistry and Western blot analysis in developing human neocortex and adult brain cortex ( n = 12 samples from first, second, and third trimesters and n = 3 adult normal cerebral cortex). Our results suggest that VASP showed different immunostaining patterns between cerebral cortical plates in prenatal and adult human brain samples. We observed that the expression patterns of the VASP protein are clearly identified in fibers, cytoplasm of neural cells and endothelial cells of vessels. We detected that VASP indicates progressive expression from the adult brain to second trimester neocortexes. Therefore, we suggest that VASP may play a crucial role in the regulation of human neonatal cerebral cortical development.