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[P1.76]: LHX7 is required in mouse striatal cholinergic interneurons to maintain neuronal subtype identity
Author(s) -
Lopes R.,
Pachnis V.
Publication year - 2010
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2010.07.116
Subject(s) - citation , lopes , library science , neuroscience , identity (music) , medicine , psychology , computer science , art , physics , quantum mechanics , aesthetics
reciprocal functions in neural precursor populations: with E2f3a regulating the stem cell pool and E2f3b modulating progenitor proliferation. This intriguing result prompted us to ask which genes are regulated by E2f3 in neural precursors, and can regulation of distinct target genes explain how E2f3 isoforms mediate their unique functions. We employed ChIP-on-chip technology to determine the target genes of E2f3a and E2f3b in neural precursors. We found that E2f3b binds a unique set of gene promoters with enrichment in cell cycle control functions. Alternatively, both isoforms bind an overlapping set of genes involved in classical and novel E2f directed activities, most markedly neurogenesis and CNS development, including multiple genes known to regulate neural precursor renewal, proliferation, differentiation, death, neuronal maturation, and growth factor responsiveness. Finally, bioinformatic analysis of transcription factor binding sites enriched in E2f3 binding regions suggests that E2f3 regulates neural precursor function in cooperation with important tissue specific and epigenetic regulators. Supported by: CIHR operating grant to RSS, CIHR CGS award to LMJ.