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[P1.60]: Transgenerational transfer of ADHD‐like phenotypes in prenatally ethanol‐exposed Sprague–Dawley rat offsprings
Author(s) -
Kim P.,
Choi I.,
Kim M.K.,
Ryu J.H.,
Shin C.Y.
Publication year - 2010
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2010.07.100
Subject(s) - transgenerational epigenetics , mathematics , genetics , biology , pregnancy , offspring
Ethanol exposure during gestational period can result in developmental, neurobehavioral and morphological abnormalities on offspring. We investigated whether maternal preconceptional ethanol intake shows ADHD-like phenotype in offspring rats. Sprague-Dawley female rats were treated with 6 g/kg/day ethanol for 10 days and mated with normal male rat at 8 weeks later. Litters were tested for their ADHD-like phenotypes using openfield, Y maze and electro-foot shock aversive water drinking test to screen behavioral abnormalities. Western blot and immunohistochemical staining were used to identify changes of dopamine and norepinephrine transporter in the brain. Offspring rats exposed to preconceptional ethanol displayed hyper-locomotive activity, attention deficit and impulsive behavior. The expression of dopamine transporter was inhibited in the striatum and also in cortex to a lesser extent. On the contrary, the expression of norepinephrine transporter was increased in the cortex and striatum. These results suggest that maternal preconceptional ethanol exposure induces ADHD-like behaviors and abnormal neural activities of catecholaminergic nervous system in SD rats.