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[P1.38]: AF9/MLLT3 interferes with TBR1 expression through epigenetic modification of histone H3K79 during development of the cerebral cortex
Author(s) -
Vogel T.,
Buettner N.,
Johnsen S.,
Kuegler S.
Publication year - 2010
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2010.07.079
Subject(s) - epigenetics , neuroscience , cerebral cortex , histone , psychology , chemistry , biochemistry , gene
cursors, from a renewable source of stem cells – embryonic stem (ES) cells. First, we will mimic, in culture, the inner ear microenvironment supportive of HC generation, taking advantage of the recent breakthroughs on the knowledge of the molecular mechanisms regulating embryonic HC development. In order to achieve this goal, we are engineering mouse ES cells to bear various fluorescent reporters under control of genes known to be expressed at specific phases of HC development, such as, for example, Sox2, Pax2, Atoh1 or Myo7A. These multi-reporter ES cell lines will allow us not only to monitor with great precision the various steps involved in HC commitment and differentiation, but also to quickly screen for the best culture conditions that result in efficient generation of sensory HCs suitable for transplantation. In a second step of this work we will proceed to transplantation studies in animal models of human deafness. We hope that this work will result in crucial data, needed to achieve the final goal of restoring auditory function by replacing damaged or lost sensory HCs.