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Neonatal LPS injection alters the body weight regulation systems of rats under non‐stress and immune stress conditions
Author(s) -
Iwasa Takeshi,
Matsuzaki Toshiya,
Kinouchi Riyo,
Fujisawa Shinobu,
Murakami Masahiro,
Kiyokawa Machiko,
Kuwahara Akira,
Yasui Toshiyuki,
Irahara Minoru
Publication year - 2010
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2009.08.015
Subject(s) - immune system , stress (linguistics) , body weight , endocrinology , medicine , immunology , linguistics , philosophy
It has been reported that prenatal immune stress induced by lipopolysaccharides or cytokines increases food intake and leads to obesity and other features of metabolic syndrome in adulthood. Using Sprague–Dawley rats, we evaluated whether neonatal LPS injection altered their body weight regulation systems under non‐stress and immune stress conditions. On Day 10 after birth, all pups were injected with LPS (100 μg/kg, i.p.) (PND 10 LPS) or saline (PND 10 Saline). After weaning, body weight was significantly elevated in PND 10 LPS compared with PND 10 Saline. Thereafter, the rats were injected with LPS (100 μg/kg, i.p.) or saline (used as a basal condition) from 7 to 8 weeks of age. Under basal conditions, cumulative food intake were significantly higher, serum leptin concentration was significantly increased, and hypothalamic NPY mRNA expression was significantly decreased in PND 10 LPS compared with PND 10 Saline. Under adult LPS injected conditions, body weight gain and cumulative food intake were suppressed in both the PND 10 LPS and PND 10 Saline groups compared with those observed under basal adult saline‐injected conditions. The suppressive effects induced by adult LPS injection were less evident in the PND 10 LPS group than in the PND 10 Saline group. Adult LPS injection increased the serum leptin concentration in the PND 10 Saline rats, but not in the PND 10 LPS rats. In addition, adult LPS injection increased the mRNA expression of anorexinergic factors (IL‐1β, and TNF‐α), and decreased that of the orexinergic factor NPY in both groups. However, the influence of adult LPS injection upon these factors was less evident in the PND 10 LPS group than in the PND 10 Saline group. These results suggest that neonatal LPS injection alters body weight regulation under both non‐stress and immune stress conditions in male rats. Changes in the endocrine, neuropeptide, and cytokine regulation systems might be involved in these alterations.