Premium
[P2.61]: Activity‐dependent specification of dopaminergic neurons in the embryonic nervous system
Author(s) -
VelázquezUlloa N.A.,
Spitzer N.C.,
Dulcis D.
Publication year - 2008
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2008.09.186
Subject(s) - citation , library science , neuroscience , computer science , humanities , cognitive science , psychology , art
Lowdosewhole-bodyg-irradiation is recently reported to confer neuroprotection against MPTP induced dopaminergic neuron toxicity. Here, we investigated whether the different low dose whole-body g-irradiation has the similarly neuroprotection. We used three different low dose g-irradiation, 0.5 Gy, 2.0 Gy and 3.5 Gy. After pretreatment of a single low dose whole-body girradiation, four times of MPTP (15 mg/kg, four times, 2 h apart) wereadministered intraperitoneally (i.p.) inmice. Lymphocytewere counted before and after the g-irradiation. On the seventh day after the last MPTP treatment, mice were killed and HPLC determination of striatal dopamine and immunostaining for tyrosine hydroxylase (TH), CD11b and GFAP to detect dopamine neurons and associated glial reaction in the substantia nigra pars compacta (SNpc) were performed. TheHPLC result suggested that 2.0 Gyg-irradiationmay have a preventive effect inMPTP-inducedmodel of PD, but themore lowerdoseg-irradiationmayhavenoneuroprotectionand0.5 Gygirradiation maybe enhance dopaminergic neuron toxicity. And 3.5 Gyg-irradiationmayhavenopreventiveeffect.At thesametime, lymphocyte decreased significantly after 3.5 and 2.0 Gy g-irradiationbutnot after 0.5 Gyg-irradiation. Therewasnodifference in the number of TH positive neurons between irradiated and control MPTP treated mice. Irradiation also did not have obvious influence onmicrogliosis and astroglial reaction induced byMPTP treatment. In conclusion, not all low-dose whole-body g-irradiation renders neuroprotection against MPTP-mediated damage of dopaminergic neuron. The protection is maybe related to the decrease of lymphocyte. This study was supported by the Major State Basic Research Development Program of China (973 Program, no. 2003CB515300).