Premium
[P2.49]: Proper dendritic morphogenesis requires KCC2 expression in immature dentate granule cells
Author(s) -
Ichikawa J.,
Matsuki N.,
Koyama R.
Publication year - 2008
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2008.09.174
Subject(s) - citation , granule (geology) , library science , neuroscience , biology , computer science , paleontology
axons from the explant exhibited radial outgrowth into thematrix. However, the explants were co-cultured with the notochord explants, growing Cnt1-positive spinal axons were repelled by notochord-derived repulsive activity. This repulsive activity was abolished by the addition of either a monoclonal anti-Cnt1 antibody, chondroitin sulfate A or chondroitinase ABC to the culturemedium. An antibody that recognizes chondroitin sulfate A and C labeled immunohistochemically the notochord in embryo sections and the collagen gelmatrix around the cultured notochord explant. Addition of chondroitinase ABC into the culture eliminated the immunoreactivity in the gel matrix. These results suggest that the notochord-derived chondroitin sulfate proteoglycan acts as a repulsive signaling molecule that is recognized by Cnt1 on spinal sensory axons. This guidance mechanism would be involved in navigation of the growing sensory axons not to enter mid-body areas where the notochord exist, resulting in their subepidermal extension.