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Effects of Ginkgolide B on 6‐OHDA‐induced apoptosis and calcium over load in cultured PC12
Author(s) -
Meng Haiwei,
Li Chuangang,
Feng Lei,
Cheng Baohua,
Wu Fengxia,
Wang Xiaohong,
Li Zhenping,
Liu Shuwei
Publication year - 2007
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2007.09.010
Subject(s) - apoptosis , calcium in biology , ginkgo biloba , neuroprotection , intracellular , chemistry , calcium , pharmacology , microbiology and biotechnology , biology , biochemistry , organic chemistry
Ginkgolide B, one of the major components of Ginkgo biloba extracts, is a potent platelet‐activating factor (PAF) receptor antagonist, which is also regarded as having neuroprotective effects on the CNS. The aim of this research is to observe the effects of Ginkgolide B on the PC12 apoptosis induced by 6‐hydroxydopamine (6‐OHDA) and to explore whether these effects are related to the changes of intracellular Ca 2+ and Calbindin D28K mRNA in PC12 cells. In the present work, the damage of PC12 cells was induced by 100 μM 6‐OHDA. The cells survival rate was examined by MTT assays. The intracellular free calcium concentration in PC12 cells was measured by using the fluorescent Ca 2+ indicator fluo‐3/AM. Semi‐quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) was employed to determine the expression of Calbindin D28K mRNA in PC12. The data show that the Ginkgolide B inhibited PC12 cells apoptosis induced by 6‐OHDA in a dose‐dependent manner, and decreased the activity of caspase‐3. In addition, Ginkgolide B increased the expression of Calbindin D28K mRNA and inhibited 6‐OHDA‐induced elevation in the intracellular calcium concentration. Our results showed that the Ginkgolide B inhibited the apoptosis of PC12 induced by 6‐OHDA, and the protective effects of Ginkgolide B on PC12 cells are mediated, at least in part, by up‐regulating the Calbindin D28K mRNA and by decreasing the intracellular calcium concentration.