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Time‐dependent changes in distribution of basic fibroblast growth factor immunoreactive cells in hippocampus after kainic acid injection in rat pups
Author(s) -
Erkanli Gozde,
Ercan Feriha,
Sirvanci Serap,
Salik Emsal,
Yananli Hasan Raci,
Onat Filiz,
San Tangul
Publication year - 2007
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2007.06.001
Subject(s) - basic fibroblast growth factor , kainic acid , cresyl violet , gliosis , hippocampus , endocrinology , medicine , epileptogenesis , immunohistochemistry , chemistry , biology , growth factor , neuroscience , pathology , glutamate receptor , staining , receptor
Five‐day‐old Wistar albino rats were injected with kainic acid (KA) or saline i.p. to investigate time‐dependent alterations in morphology and number of basic fibroblast growth factor (bFGF) immunoreactive (‐ir) astrocytes and neurons in hippocampus at 15, 30, and 90 days after the injections. Sections were stained with cresyl violet for morphological evaluation and bFGF immunohistochemistry was used for quantitative evaluation of bFGF‐ir cell density. Fifteen days after KA injection, there was gliosis but no neuronal loss although disorganization in CA1, CA3, CA4 pyramidal layers and neuronal loss were evident 30 and 90 days after the injection. KA injected rats demonstrated significantly increased number of bFGF‐ir astrocytes throughout the hippocampus and pyramidal neurons in CA2 after 15 days and decreased number of bFGF‐ir cells after 30 and 90 days. The decrease in the number of bFGF‐ir astroglia and neurons in long term after KA injection may indicate a decrease in the production of bFGF and/or number of bFGF‐ir cells suggesting that protective effects of bFGF may be altered during epileptogenesis in hippocampus.