Premium
[P130]: Early specification of dopaminergic phenotype during ES cell differentiation
Author(s) -
Parmar M.,
Li M.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.192
Subject(s) - citation , phenotype , library science , stem cell , neuroscience , psychology , biology , computer science , genetics , gene
is suggested since treatment of dorsal-neural progenitor cells, in culture, with fibroblast growth factor 2 (FGF-2) results in OLP induction. To ask if dorsal induction of OLPs can occur in vivo, and if FGF-2 could initiate an alternative pathway of regulation, we used in utero microinjection of FGF-2 into the lateral ventricles of mouse fetal forebrain. A single injection of FGF-2 at E13.5 resulted in expression of the OLP markers Olig2 and PDGFRα mRNA in dorsal forebrain ventricular and intermediate zones. However, FGF-2 did not induce dorsal expression of Shh, Patched1 or Nkx2.1, suggesting that Shh signaling was not involved in this FGF-2 mediated dorsal induction. These results demonstrate that the dorsal forebrain in vivo has the potential to generate OLPs in the presence of normal positional cues, and that this can be driven by FGF-2 independent of Shh signaling.