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[P128]: Role of two Alzheimer's disease protein markers: Amyloid precursor protein and acetylcholinesterase with aggression in autism
Author(s) -
Sokol D.,
Maloney B.,
Kardatzke D.,
Chen D.,
Lahiri D.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.190
Subject(s) - library science , citation , aggression , psychology , medicine , gerontology , computer science , psychiatry
The results revealed that, the proneural domain is characterized by the expression of Sox3 and Fgf10, while the non-neural domain by Irx1, Lmx1. We performed cell labeling experiments (with two different vital fluorescent dyes) in ovo to analyze whether the two complementary otic gene expression domains exhibited restricted cell mingling. The results showed that proneural and non-neural territories presented restricted cell mixing. Cell labeling and in situ hybridization experiments, showed that labeled clones exhibited an antero-posterior boundary that was coincident with the Fgf10/Hairy1 expression limits. These results suggest that, in order to give rise different cell types, during early stages of the inner ear development the otic territory is segregated into two cellular units that will develop independently. We present data showing that the elements of Notch pathway, one of the pathways that can govern cell fate decisions and regionalization, were complementary expressed in the anterior and posterior territories: Delta1, LFng and Hes5 in the proneural, and Serrate1 and Hairy1 in the non-neural indicating that Notch operates in both domains. Blockade of Notch signaling by the gamma-secretase DAPT, was used to assess the function of Notch in early otic regionalization. The results showed an increase of neuroblasts population in the anterior domain without effecting anterior regionalization. Surprisingly, Lmx1 and Irx1, after Notch blockade were also expressed in the anterior domain. Expansion of posteriors markers was not due to cell migration of posterior cells but to regulation on gene expression. In summary, we have seen that Notch signaling mediates two distinct roles during early stages of inner ear development: (1) in the anterior domain regulates neurogenesis by lateral inhibition and (2) it is also required for downregulating Irx1 and Lmx1 in the anterior domain.