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[P123]: Characterization of rat rostral raphe primary cultures: A model for examining pre‐synaptic serotonergic neurons
Author(s) -
Czesak M.,
Burns A.,
Lenicov F. Remes,
Albert P.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.185
Subject(s) - serotonergic , citation , library science , raphe , neuroscience , psychology , medicine , computer science , serotonin , receptor
the present study, we identify a new DRE in the second intron of the dopamine D2 receptor gene that binds to Freud-1 in vitro. Using chromatin immunoprecipitation we demonstrate an in vivo interaction between endogenous Freud-1 and the DRE elements in both 5-HT1A and dopamine-D2 genes. Knockdown experiments using siRNA of Freud-1 demonstrate up-regulation of dopamine-D2 RNA, particularly in cells that express low levels of the receptor. We conclude that Freud-1 negatively regulates both 5-HT1A and dopamine-D2 receptor expression, and hence may influence the development and activity of serotonin and dopamine systems. Further studies will identify the importance of Freud-1 in developmental regulation of 5-HT1A and dopamine-D2 genes and how Freud-1 induced regulation leads to developmental disorders, such as mental retardation, autism, schizophrenia or depression.