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[P120]: Coordinated actions of Hes1, Hes5, Hes6 and NUDR on 5‐HT1A transcription at a functional polymorphism
Author(s) -
Jacobsen K.,
Albert P.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.182
Subject(s) - hes1 , library science , citation , transcription (linguistics) , computer science , biology , genetics , philosophy , gene , linguistics , notch signaling pathway
the G-protein coupled receptors CXCR1 and CXCR2. However, the widely distribution of CXCL8 and its both receptor expression in several tissues and cell types suggests other functions beyond the regulation of leukocyte migration, e.g. angiogenesis, tumor growth or brain pathology. Several diseases of the eye were accompanied by inflammatory processes and migration and/or proliferation of neuroglia cells. Therefore, we examined the expression of CXCL8 and its receptors in human retinal glial cells. The primary cultured cells from human donor retinas were mainly immunoreactive for glia fibrillary acidic protein (GFAP) vimentin, S-100, S-100 and cellular retinaldehyd binding protein (CRALBP) and represent cell cultures of macroglial cells, i.e. astrocytes and Müller glial cells. Immunoreactivity for CXCL8, CXCR1 and CXCR2 could be observed in virtually all cultured glial cells. The expression of CXCL8, CXCR1 and CXCR2 was confirmed in immortilized human Müller cell line (MIO-M1) by immunohistochemistry, RT-PCR analysis and Western blotting. Calcium imaging experiments revealed functional CXCL8 receptors in Müller cells after stimulation with recombinant CXCL8. To our surprise, we could detect CXCR1 and CXCR2 expression in normal retinae of human organ donors by means immunohistochemistry. RT-PCR analysis and Western blotting. This suggests additional function of CXCL8 receptors in healthy retinal tissues beyond the involvement in pathological processes. Because of several similarities of cultured glial cells and gliotic glial cells of the retina and the expression of CXCL8 and both of its functional receptors, Müller glial cellsand asrocytes may participate in the inflammatory response of the retina in diseases of the eye.