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[P110]: Identification of genes necessary for the development of cranial motor neurons
Author(s) -
Walsh G.S.,
CarmanyRampey A.,
Grant P.K.,
Wark A.,
Margaretha L.,
Moens C.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.172
Subject(s) - hindbrain , zebrafish , biology , neuroscience , rhombomere , mutant , green fluorescent protein , neuron , homeobox , anatomy , lateral line , microbiology and biotechnology , central nervous system , hox gene , gene , genetics , gene expression
glial cells are obtained from E16 cerebellum and, when grown in the presence of FGF and EGF, assume a bipolar morphology and maintain a very immature phenotype, expressing typical progenitors markers, such as Nestin, RC2, Glast and BLBP. Astrocytes are obtained from P3 cerebellum at a time when all radial glial cells have translocated to the Bergmann glia formation. Those cells grow slowly, express GFAP and assume a flat epithelioid morphology. A comparison of the proteomes of the two cell types has been realized using the 2D-DIGE technology, which allows a very precise and sensitive determination of differential protein expression. Statistical analysis of the protein spots distribution and abundance revealed 222 and 128 protein spots that were increased >1.5-fold in radial glia and astrocytes, respectively. After tryptic digestion and Maldi-TOF sequencing, 88 and 60 unique proteins were identified as being expressed between 1.5and 29-fold more in radial glia and astrocytes respectively. Radial glial cells express in particular a large array of chaperones, including 7 forms of the T-complex protein 1, 17 different cytoskeletal proteins including 4 dihydropyrimidinaserelated proteins and 4 tubulin chains, several enzymes of lipid metabolism, signaling molecules, such as FABP and Immunophilins, several AA-tRNA synthase and 8 different splicing-involved hnRNPs. Astrocytes generally express more mature forms of chaperones and cytoskeletal proteins, such as Actin, GFAP, Moesin, Vimentin and Vinculin, enzymes of intermediary metabolism, Peroxiredoxins and 5 forms of Annexins. Information from this study will be useful to uncover the molecular mechanisms of astroglial differentiation and maturation.