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[P98]: Biomarker discovery for polyglutamine‐mediated SCA17 using transiently transfected 293 and lymphoblastoid cells with TBP expansion
Author(s) -
Lee L.C.,
Chen F.L.,
Chen C.M.,
Chang K.H.,
LeeChen G.J.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.160
Subject(s) - chen , library science , theology , medicine , philosophy , biology , computer science , paleontology
cells (FS) (Inoue et al., 1999). During early postnatal development, rat anterior pituitary cells re-accommodate in the gland tissue, implicating intense migration activity. We have shown that cultured infantile pituitary cells migrate preferentially over collagen type I/III, and the epidermal growth factor (EGF) stimulates this behaviour. Moreover, EGF stimulation induces an increase in cell association (González et al., 2004) and cell spreading (Toral et al., 2003). The aim of the present study was to investigate more about the migration and association behaviour of SC and FS using a vertical diffusion chamber. Aliquots of 3 × 10 rat infantile pituitary cells were placed in the upper compartment without or with EGF (50 ng/ml) in a defined culture medium. Cells were allow migrating to the lower compartment through the 12 m pore size and settled on a cover glass coated with collagen I/III. After 48 h, the migrated cells were fixed and stained with toluidine blue or assay for fluorescent-immunocytochemistry for S-100 , identifying FS, chromogranin A, identifying SC, nucleus with To-Pro-3iodide and F-actin with TRICT-phalloidin. Cells were visualized with a light microscope for cell number measurement, or with a confocal microscope. When cells were stimulated with EGF an increase in cell migration and cell association were observed. In FS, S-100 was observed like fibres crossing the cell body and in membrane outgrowth processes. These membrane extensions were directed to other FS. When both cell types were forming clusters, S-100 fibres were observed like involving the cluster. Also, a thin S100 band was seen in the boundary between FS and SC. These data suggest a participation of S-100 in the mechanism of cell interaction between FSs and with SCs in the cluster formation.