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[P94]: Class 3 semaphorins expressed in motoneurons influence angiogenesis in the developing zebrafish
Author(s) -
Lamont R.,
Childs S.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.156
Subject(s) - semaphorin , class (philosophy) , zebrafish , citation , library science , editorial board , biology , cognitive science , neuroscience , computer science , psychology , genetics , artificial intelligence , gene , receptor
and elaboration of neuronal networks. Therefore, we chose the embryonic CNS as a model for examining mechanisms that coordinately regulate angiogenesis and neurogenesis. Methods: S-phase labeling methods were used to study cell proliferation; laser capture micro-dissection and real-time quantitative PCR to study gene expression profiles of angiogenesisrelated genes in neurogenic regions of the telencephalon. Results: Endothelial and neuroepithelial cell proliferation is concurrently active in the proliferative zones of the telencephalon early in embryonic development. However, later in embryonic development, endothelial cell proliferation continues in postmitotic domains independent of neuroepithelial cell proliferation. BrdU labeling indices in the ventricular and subventricular zones decline coordinately in endothelial and neuroepithelial cells from embryonic day 11 (E11) to E17. Differences between dorsal and ventral telencephalon were apparent in endothelial cell BrdU labeling patterns. Novel patterns of VEGF, Flt1, Flk1, Tie1 and Tie2 mRNA expression emerged in endothelial and non-endothelial cells in the proliferative zones from E11 to E17. Discussion: Our data lay the foundations for a comprehensive model of vascular and nervous system development and offer insights into molecules and mechanisms that integrate development and repair of both the systems.