[P83B]: Bioelectric properties of developing neuroectodermal stem cells

In about 50% of non-differentiated pass-cells, blocking GJs resulted in the appearance of outwardly rectifying VD currents. These VD currents were only partially inhibited by tetraethylammonium (TEA, 5mM) but were completely blocked by 2 mM 4-AP. In addition to passive conductance, KDR currents were also reduced by GRA in non-differentiated pass+VD cells. Residual VD currents were not or only partially reduced by TEA, but were completely blocked by 4-AP. The pharmacological properties of KDR changed during development. In non-differentiated pass+VD cells KDR currents were only partially reduced by TEA, while 4-AP almost completely diminished the current. In NE-4C neurons, on the other hand TEA exerted a more profound (~60 %) blocking effect. Inhibiting KDR by TEA and 4-AP