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[P46]: Cortical stem cells coexist with striatal stem cells in the adult lateral ventricular subependyma, but give rise to distinct progeny in the olfactory bulb and striatum
Author(s) -
WillaimeMorawek S.,
Seaberg R.M.,
Jones K.R,
Kooy D.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.109
Subject(s) - olfactory bulb , citation , striatum , neuroscience , stem cell , psychology , biology , library science , computer science , genetics , dopamine , central nervous system
Cortical neural stem cells (NSCs) can be isolated from the embryonic cortical germinal zone but do not persist in the adult cortex. In contrast, NSCs from the striatal embryonic germinal zone persist in the adult lateral ventricular subependyma. To permanently mark and follow the fate of all cortical stem cells that express Emx1 at any point during development, we used Emx1IRES-Cre mice mated to the Cre-dependent reporter strain Z/EG. These mice revealed that GFP+ embryonic cortical NSCs survive and migrate ventrally into the perinatal subependyma. Cutting the connections between cortex and striatum in slice cultures prevented the migration of Emx1-lineage cells into the striatal germinal zone. Thus, embryonic Emx1-lineage NSCs migrate ventrally into the striatal germinal zone to coexist with the resident population of striatal NSCs and persist into adulthood. To analyze the fate of the Emx1-lineage cells in the subependyma, BrdU injections were performed to label proliferating cells: this analysis reveals that GFP+, cortically derived striatal cells proliferate faster than host striatal GFP− cells in the adult lateral ventricular subependyma. Following GFP+ cells migrating in the rostral migratory stream to the olfactory bulb in the adult, reveals that GFP+ cells, like GFP− cells, make gabaergic and calretinin+ neurons in granular and glomerular layers and dopaminergic neurons in the glomerular layer. However, GFP+ cells make more new cells than GFP− cells in the adult glomerular layer. Perinatally, GFP+ cells in the striatal germinal zone also give rise to cells in the striatum. Like GFP− cells, they make astrocytes, as well as gabaergic, DARP32+, calbindin+, and calretinin+ neurons. However, GFP+ cells give rise to more astrocytes and less neurons than GFP− cells in the striatum. In conclusion, Emx1-lineage (neocortically derived) NSCs give rise to cells in the olfactory bulb and striatum, but in different proportions than do resident striatal germinal zone cells.