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[P35]: Role of dbpA in neural crest and Schwann cell development
Author(s) -
Castagner F.,
Atanasoski S.,
Balda M.,
Matter K.,
Suter U.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.099
Subject(s) - citation , library science , neural crest , art history , computer science , art , chemistry , biochemistry , gene
and methods: Olfactory epithelium (OE) consists of multiple cell types such as olfactory neurons, supporting cells, gland cells and basal cells and is derived from nasal placodes. Six1, a member of Six homeobox gene family, is first expressed in the nasal placodes and continues to be expressed throughout the OE development in mice. To understand how Six1 is involved in OE development, we analyzed Six1-deficient homozygous mice (Six1) by in situ hybridization with probes of various olfactory neuron marker genes as well as by immunohistochemistry. We also assessed the effects of Six1 on neural differentiation using P19EC cells. Results: Six1 showed defects in differentiation of olfactory neurons, supporting cells and gland cells. The expression of proneural marker Mash1 was unchanged, while those of Ngn1, NeuroD and Lhx2 were severely reduced at E10.5. Olfactory neuron specific marker protein OMP was missing in the Six1 at E16.5. The expression of Six1 was unaltered in the olfactory epithelium of Mash1 mice, indicating that Six1 and Mash1 regulate OE differentiation independently. The expressions of Hes1 and Hes5 in OE were augmented in the Six1 at E10.5. No supporting cells were observed in Six1 at E18.5. The expression of Ngn1, Mash1 and Hes1 upon neural differentiation induction of P19 cells was altered when Six1 was constitutively overexpressed. Discussion: Six1 is involved in differentiation of olfactory neuron and supporting cells in OE through regulation of Ngn1, NeuroD, Lhx2, Hes1 and Hes5.