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[P13]: Effects of aluminum chloride on Na + current and transient outward K + current and delayed rectifier K + current in acutely isolated rat hippocampal CA1 neurons
Author(s) -
Meng Z.,
Nie A.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.077
Subject(s) - current (fluid) , hippocampal formation , physics , computer science , library science , neuroscience , psychology , thermodynamics
zone (SGZ) of the dentate gyrus (DG). Here we investigated the effects in neurogenesis in the SVZ after chromaffin cells transplant in animal model of Parkinsons disease. Methods: Male Wistar rats were subjected to the unilateral destruction of the substantia nigra by applying 6-OHDA and transplanted with neonatal chromaffin cells. Chromaffin cells were stained prior to implant with FluoroGold. Intraperitoneal injections of BrdU (50 mg/kg) were given twice daily during days 44–45 after transplant. Animals were killed at 2 wk after the last BrdU injection. Results: The results obtained were: (1) improvement in motor deficits and an over expression of Tyrosine Hydroxilase (TH) positive cells in both lateral ventricles of the nigro-striatal lesioned rats with chromaffin cell transplant; (2) no migration of implanted cells and differentiation of progenitor cells of both subventricular zone. Most cells immunoreactive both to TH and to BrdU were observed in the SVZ. A few scattered doubly labeled cells were also found in the striatum adjacent to the ventricle wall. It is tempting to speculate that these cells originated in the SVZ and migrated into the denervated striatum nearby. Discussion: The induction of neurogenesis in the SVZ following SN lesions or grafts of chromaffin cells has not been reported (Arias-Carrión et al., 2004). Destruction of the SN and the ensuing dopaminergic denervation of the striatum can induce trophic signals, similar to those released after ischemia, leading to increased neurogenesis. Similarly, augmented proliferation and differentiation in the SVZ subsequent to grafts of chromaffin cells could result from (1) production of local trophic signals by the injury of grafting itself and (2) local release of neurotrophic molecules known to be present in chromaffin cells granules (Krieglstein and Unsicker, 1997). Admittedly, the effect of these factors should be more noticeable ipsilateral to the denervation site/graft. However, similar neurogenesis took place in the SVZ of both lateral ventricles, suggesting a widespread distribution of these signal(s). The reasons for this bilateral pattern must be investigated further.