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[P4]: Analysis of cell proliferation in the mouse brain upon the adenovirus‐mediated transfer of sonic hedgehog and hedgehog interacting protein
Author(s) -
Angot E.,
Loulier K.,
Traiffort E.,
Ruat M.
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.09.068
Subject(s) - hedgehog , sonic hedgehog , microbiology and biotechnology , biology , genetics , gene
Cells expressing Tbr2 were localized to the SGZ, and exhibited morphology typical of intermediate stage progenitors. Colabeling with PCNA, a marker of proliferating cells, showed that 92.61 ± 3.79% of Tbr2-positive cells were also PCNA-positive. Acute bromodeoxyuridine labeling showed that 34.40 ± 5.43% of Tbr2-positive cells were in S-phase of the cell cycle at the time of labeling. A subset of Tbr2–positive cells colocalized with Pax6 (35.67 ± 1.67%), similar to the expression patterns observed for these TFs during embryonic cortical neurogenesis. Tbr2-positive cells coexpressed different markers of intermediate stage progenitors, including Doublecortin (64.35 ± 4.67% of Tbr2-positive cells) and PSA-NCAM. Studies of Tbr2 (Eomes)EGFP transgenic mice showed that Tbr2-positive cells differentiate into Prox1-positive GCs. Tbr1 was expressed by most post-mitotic granule neurons and its expression was strongest in cells that colocalize with markers of immature neurons, such as NeuroD. Therefore, the current evidence indicates that Tbr2 is expressed specifically in intermediate stage progenitors, while Tbr1 expression is upregulated in newly generated GCs.