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Endogenous semicarbazide‐sensitive amine oxidase (SSAO) inhibitor increases 1‐methyl‐4‐phenylpyridinium ion (MPP + )‐induced dopamine efflux by immobilization stress in rat striatum
Author(s) -
Obata Toshio
Publication year - 2006
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2006.04.002
Subject(s) - chemistry , dopamine , semicarbazide , endogeny , striatum , efflux , monoamine oxidase , biophysics , biochemistry , enzyme , endocrinology , biology , organic chemistry
The present study examined whether or not immobilization stress (IMMO)‐inducible semicarbazide‐sensitive amine oxidase (SSAO) inhibitor by separated gel filtration from 105,000 g supernate in rat brain cytosol contribute to the dopamine (DA) efflux by 1‐methyl‐4‐phenylpyridinium ion (MPP + ) in the rat striatum. The isoelectric point (p I ) value of this inhibitor was determined by isoelectric focusing (IEF)‐gel electrophoresis to about 3.8. The application of IMMO‐induced SSAO inhibitor (3 μg) by IEF‐gel electrophoresis increased DA efflux by MPP + in rat striatum. These results suggest that IMMO‐inducible endogenous SSAO inhibitor enhances DA efflux by MPP + .