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Spatio‐temporal expression of immediate early genes in the central nervous system of SJL/J mice
Author(s) -
Gerhauser Ingo,
Alldinger Susanne,
Ulrich Reiner,
Baumgärtner Wolfgang
Publication year - 2005
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2005.06.004
Subject(s) - biology , cerebellum , central nervous system , cerebrum , spinal cord , gene expression , granule cell , purkinje cell , in situ hybridization , cerebral cortex , microbiology and biotechnology , gene , pathology , neuroscience , genetics , dentate gyrus , medicine
Gene products of immediate early genes (IEGs) interact with specific binding sites in promoter regions of inducible and constitutively expressed genes. Thereby, they control transcription of down‐stream targets, like pro‐ and anti‐apoptotic genes and matrix‐metalloproteinases (MMPs), known to play an important role in development, plasticity, response to injury and repair of the central nervous system (CNS). A real‐time quantitative RT‐PCR and immunohistochemical investigation was performed to study mRNA expression levels and protein distribution patterns of IEGs in cerebrum, cerebellum, and spinal cord of SJL/J mice between postnatal weeks 1 and 40. A down‐regulation of c‐jun, NF‐κB1, Max, Ets‐1, and p53 mRNA, and an up‐regulation of c‐fos mRNA was noticed. Down‐regulations of Ets‐1 and p53 were most prominent between week 1 and 3. The prominent role in CNS development for c‐jun, Ets‐1 and Max was supported by immunohistochemistry. One‐week‐old mice were strongly positive for all three proteins in cerebral cortex, medulla oblongata, and gray matter of the spinal cord. A high staining intensity was detected in the developing granule cell layer of the cerebellum for c‐jun and Ets‐1, and in the Purkinje cell layer of the cerebellum for Max. In addition to the general down‐regulation of most mRNAs, minor up‐regulations of all IEG proteins could be detected in restricted parts of the CNS indicating regional variations and differential expression and translation during development. Apoptosis was demonstrated using immunohistochemistry for active caspase‐3. The expression patterns of IEGs might represent the key to understand the balance of proteolytic activities by MMPs, myelination, and the induction of apoptosis during the development of the CNS.