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Analysis of age dependent changes of Topoisomerase II α and β in rat brain
Author(s) -
Kondapi Anand K.,
Mulpuri Neelima,
Mandraju R.K.,
Sasikaran B.,
Subba Rao K.
Publication year - 2004
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/j.ijdevneu.2003.10.006
Subject(s) - topoisomerase , gene isoform , cerebellum , biology , embryo , beta (programming language) , ageing , alpha (finance) , endocrinology , microbiology and biotechnology , medicine , enzyme , biochemistry , genetics , gene , construct validity , nursing , computer science , programming language , patient satisfaction
Eukaryotic Topoisomerase II (Topo II) is present in two isoforms α and β. The α isoform is predominantly localized in proliferative tissue, while β isoform is present in all tissues. In the present study we report the activity and protein levels of Topoisomerase II α and β in rat brains of different age groups viz.: E11 (Embryo day 11), E18 (Embryo day 18), post‐natal day 1, young (<10 days), adult (<6 months) and old (>2 years). Topoisomerase II β isoform is found to be the predominant form in brain tissue but Topoisomerase II α is found in embryos up to post‐natal day 1. The studies to examine the regional distribution of Topoisomerase II β in brain showed highest activity in cerebellar region and that too only neuronal cell fraction. There was a significant age‐dependent decline in this activity. Hence, Topoisomerase II β may have some unknown function in cerebellum and the low levels of Topoisomerase II β activity in ageing cerebellum may contribute to the genomic instability in cerebellar region of ageing brain.