Open AccessIn vivo assay to monitor flavonoid uptake across plant cell membranes
Author(s) -
Filippi Antonio,
Petrussa Elisa,
Peresson Carlo,
Bertolini Alberto,
Vianello Angelo,
Braidot Enrico
Publication year - 2015
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2015.08.009
Subject(s) - flavonoid , plant cell , in vivo , quercetin , subcellular localization , vitis vinifera , chemistry , membrane , biochemistry , cell , microbiology and biotechnology , biology , botany , cytoplasm , gene , antioxidant
Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unclear. This is due to the absence of a convenient and simple method to monitor their transport. In the present work, we suggest an assay able to follow in vivo transport of quercetin, the most abundant flavonoid in plant tissues. This uptake was monitored using 2‐aminoethoxydiphenyl borate (DPBA), a fluorescent probe, in non‐pigmented Vitis vinifera cell cultures.