Open AccessFunctional classification and biochemical characterization of a novel rho class glutathione S ‐transferase in Synechocystis PCC 6803
Author(s) -
Pandey Tripti,
Chhetri Gaurav,
Chinta Ramesh,
Kumar Bijay,
Singh Dev Bukhsh,
Tripathi Timir,
Singh Arvind Kumar
Publication year - 2015
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2014.11.006
Subject(s) - glutathione , transferase , glutathione s transferase , biochemistry , chemistry , structural similarity , glutathione transferase , enzyme
We report a novel class of glutathione S ‐transferase (GST) from the model cyanobacterium Synechocystis PCC 6803 (sll1545) which catalyzes the detoxification of the water pollutant dichloroacetate and also shows strong glutathione‐dependent peroxidase activity representing the classical activities of zeta and theta/alpha class respectively. Interestingly, sll1545 has very low sequence and structural similarity with these classes. This is the first report of dichloroacetate degradation activity by any bacterial GST. Based on these results we classify sll1545 to a novel GST class, rho. The present data also indicate potential biotechnological and industrial applications of cyanobacterial GST in dichloroacetate‐polluted areas.