Open AccessIdentification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short‐term exposure to tobacco smoke
Author(s) -
Niimori-Kita Kanako,
Ogino Kiyoshi,
Mikami Sayaka,
Kudoh Shinji,
Koizumi Daikai,
Kudoh Noritaka,
Nakamura Fumiko,
Misumi Masahiro,
Shimomura Tadasuke,
Hasegawa Koki,
Usui Fumihiko,
Nagahara Noriyuki,
Ito Takaaki
Publication year - 2014
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2014.08.002
Subject(s) - phosphoprotein , nicotine , biology , tobacco smoke , phosphorylation , medicine , microbiology and biotechnology , environmental health , neuroscience
Smoking is a risk factor for lung diseases, including chronic obstructive pulmonary disease and lung cancer. However, the molecular mechanisms mediating the progression of these diseases remain unclear. Therefore, we sought to identify signaling pathways activated by tobacco‐smoke exposure, by analyzing nuclear phosphoprotein expression using phosphoproteomic analysis of lung tissue from mice exposed to tobacco smoke. Sixteen mice were exposed to tobacco smoke for 1 or 7 days, and the expression of phosphorylated peptides was analyzed by mass spectrometry. A total of 253 phosphoproteins were identified, including FACT complex subunit SPT16 in the 1‐day exposure group, keratin type 1 cytoskeletal 18 (K18), and adipocyte fatty acid‐binding protein, in the 7‐day exposure group, and peroxiredoxin‐1 (OSF3) and spectrin β chain brain 1 (SPTBN1), in both groups. Semi‐quantitative analysis of the identified phosphoproteins revealed that 33 proteins were significantly differentially expressed between the control and exposed groups. The identified phosphoproteins were classified according to their biological functions. We found that the identified proteins were related to inflammation, regeneration, repair, proliferation, differentiation, morphogenesis, and response to stress and nicotine. In conclusion, we identified proteins, including OSF3 and SPTBN1, as candidate tobacco smoke‐exposure markers; our results provide insights into the mechanisms of tobacco smoke‐induced diseases.