Open AccessAnalysis of essential pathways for self‐renewal in common marmoset embryonic stem cells
Author(s) -
Nii Takenobu,
Marumoto Tomotoshi,
Kawano Hirotaka,
Yamaguchi Saori,
Liao Jiyuan,
Okada Michiyo,
Sasaki Erika,
Miura Yoshie,
Tani Kenzaburo
Publication year - 2014
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2014.02.007
Subject(s) - embryonic stem cell , marmoset , leukemia inhibitory factor , fibroblast growth factor , microbiology and biotechnology , biology , regenerative medicine , stem cell , basic fibroblast growth factor , pi3k/akt/mtor pathway , signal transduction , growth factor , immunology , cancer research , genetics , receptor , paleontology , gene
Common marmoset (CM) is widely recognized as a useful non‐human primate for disease modeling and preclinical studies. Thus, embryonic stem cells (ESCs) derived from CM have potential as an appropriate cell source to test human regenerative medicine using human ESCs. CM ESCs have been established by us and other groups, and can be cultured in vitro . However, the growth factors and downstream pathways for self‐renewal of CM ESCs are largely unknown. In this study, we found that basic fibroblast growth factor (bFGF) rather than leukemia inhibitory factor (LIF) promoted CM ESC self‐renewal via the activation of phosphatidylinositol‐3‐kinase (PI3K)‐protein kinase B (AKT) pathway on mouse embryonic fibroblast (MEF) feeders. Moreover, bFGF and transforming growth factor β (TGFβ) signaling pathways cooperatively maintained the undifferentiated state of CM ESCs under feeder‐free condition. Our findings may improve the culture techniques of CM ESCs and facilitate their use as a preclinical experimental resource for human regenerative medicine.