Open AccessDetection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI‐SpiralTOF
Author(s) -
Nishikawa Kahoko,
Hashimoto Masahiro,
Itoh Yoshiyuki,
Hiroi Sadayuki,
Kusai Akihiko,
Hirata Fumihiko,
Sakamoto Toshihisa,
Iwaya Keiichi
Publication year - 2014
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2014.02.005
Subject(s) - mass spectrometry , chemistry , matrix assisted laser desorption/ionization , fatty liver , mass spectrometry imaging , maldi imaging , distribution (mathematics) , chromatography , desorption , medicine , mathematical analysis , mathematics , disease , organic chemistry , adsorption
Matrix‐assisted laser desorption/ionisation spiral orbit‐type time‐of‐flight mass spectrometry (MALDI‐SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m / z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m / z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI‐SpiralTOF is a powerful tool for clinical screening and estimating fatty liver.