Open AccessDifferent effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro
Author(s) -
Maciąg-Dorszyńska Monika,
Szalewska-Pałasz Agnieszka,
Węgrzyn Grzegorz
Publication year - 2013
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2013.03.001
Subject(s) - dnag , stringent response , escherichia coli , dna replication , bacillus subtilis , primase , guanosine , biology , dnab helicase , dna , biochemistry , chemistry , bacteria , eukaryotic dna replication , genetics , helicase , gene , rna , reverse transcriptase
Inhibition of Escherichia coli DNA replication by guanosine tetraphosphate (ppGpp) is demonstrated in vitro . This finding is compatible with impairment of the DnaG primase activity by this nucleotide. However, in agreement to previous reports, we were not able to detect a rapid inhibition of DNA synthesis in E. coli cells under the stringent control conditions, when intracellular ppGpp levels increase dramatically. We suggest that the process of ppGpp‐mediated inhibition of DnaG activity may be masked in E. coli cells, which could provide a rationale for explanation of differences between ppGpp effects on DNA replication in E. coli and Bacillus subtilis .