Open AccessAntimalarial drug chloroquine counteracts activation of indoleamine (2,3)‐dioxygenase activity in human PBMC
Author(s) -
Gostner Johanna M.,
Schröcksnadel Sebastian,
Becker Kathrin,
Jenny Marcel,
Schennach Harald,
Überall Florian,
Fuchs Dietmar
Publication year - 2012
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2012.08.004
Subject(s) - chloroquine , indoleamine 2,3 dioxygenase , peripheral blood mononuclear cell , neopterin , pharmacology , in vitro , immune system , drug , plasmodium falciparum , immunology , medicine , chemistry , tryptophan , malaria , biochemistry , amino acid
Antimalarial chloroquine is also used for the treatment of immune‐mediated diseases. The interference of chloroquine with interferon‐γ‐induced tryptophan breakdown and neopterin production has been investigated in human peripheral blood mononuclear cells (PBMC) in vitro . Micromolar concentrations (2–50 μM) of chloroquine dose‐dependently suppressed mitogen‐induced tryptophan breakdown in PBMC but not in the myelomonocytic THP‐1‐Blue cell line, after 48 h of treatment. In stimulated PBMC, neopterin production was super‐induced by 10 μM chloroquine, while it was significantly suppressed at a concentration of 50 μM. These anti‐inflammatory effects may relate to the therapeutic benefit of chloroquine in inflammatory conditions and may widen the spectrum of its clinical applications.