Open AccessMetabolomic profiling reveals that Drosophila melanogaster larvae with the y mutation have altered lysine metabolism
Author(s) -
Bratty Mohammed Al,
Chintapalli Venkateswara R.,
Dow Julian A.T.,
Zhang Tong,
Watson David G.
Publication year - 2012
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2012.07.007
Subject(s) - metabolomics , drosophila melanogaster , lysine , metabolism , biology , genetics , mutation , profiling (computer programming) , biochemistry , computational biology , bioinformatics , amino acid , gene , computer science , operating system
Yellow ( y ) encodes a protein which is closely similar to major royal jelly proteins produced by bees. However, the function of y remains largely unknown. Metabolomic profiling was carried out on third instar Oregon R (OR) and yellow ( y ) Drosophila melanogaster larvae. Phenylalanine, tyrosine and DOPA were all elevated in y as might be expected since the mutation blocks melanin biosynthesis. The most consistent effects were related to lysine metabolism, with the lysine metabolite saccharopine being much higher in y . In addition, lysine acetate was elevated, and the levels of methyl lysines were lower, in y than in OR.