Open AccessFormation of MgF 3 − ‐dependent complexes between an AAA + ATPase and σ 54
Author(s) -
Zhang Nan,
Buck Martin
Publication year - 2012
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2012.04.002
Subject(s) - atpase , homogeneous , transcription (linguistics) , chemistry , nucleotide , bacteria , transcription factor , biophysics , microbiology and biotechnology , biochemistry , biology , enzyme , physics , genetics , gene , linguistics , philosophy , thermodynamics
The widely distributed bacterial σ 54 ‐dependent transcription regulates pathogenicity and numerous adaptive responses in diverse bacteria. Formation of the σ 54 ‐dependent open promoter complex is a multi‐step process driven by AAA + ATPases. Non‐hydrolysable nucleotide analogues are particularly suitable for studying such complexity by capturing various intermediate states along the energy coupling pathway. Here we report a novel ATP analogue, ADP–MgF 3 − , which traps an AAA + ATPase with its target σ 54 . The MgF 3 − ‐dependent complex is highly homogeneous and functional assays suggest it may represent an early transcription intermediate state valuable for structural studies.