Open AccessGlycolytic enzyme inhibitory and antiglycation potential of rutin
Author(s) -
Shagun Dubey,
Aditya Ganeshpurkar,
Ankit Ganeshpurkar,
Divya Bansal,
Nazneen Dubey
Publication year - 2017
Publication title -
future journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2314-7253
pISSN - 2314-7245
DOI - 10.1016/j.fjps.2017.05.005
Subject(s) - rutin , chemistry , fructosamine , bovine serum albumin , enzyme , biochemistry , inhibitory postsynaptic potential , glycolysis , glycation , albumin , chromatography , insulin , antioxidant , biology , microbiology and biotechnology , receptor , neuroscience
The aim of the present was to determine the mechanism for the antidiabetic potential exerted by rutin by studying its effect on the glycolytic enzyme inhibitory and antiglycation potential. For the determination of glycolytic enzyme inhibitory potential of rutin, porcine pancreatic amylase inhibitory assay and α-glucosidase inhibitory assay were performed. The antiglycation potential was determined by glycation of bovine serum albumin followed by antiglycation estimation of fructosamines adducts, protein carbonyls, protein thiols, congo red absorbance. Rutin showed a significant inhibition of α-amylase (p < 0.001; 53.66%) and α-glucosidase (p < 0.001; 52.56%). To study antiglycation potential, various parameters were determined and fructosamine inhibition was found to be 35.55%, protein carbonyls were inhibited up to 13.49%. Protein thiols were inhibited up to 80.27%. In the present study, it was concluded that rutin showed glycolytic enzyme inhibitory and antiglycation potential