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Unusual effects of crowders on heme retention in myoglobin
Author(s) -
Kundu Jayanta,
Kar Uddipan,
Gautam Saurabh,
Karmakar Sandip,
Chowdhury Pramit K.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.11.015
Subject(s) - myoglobin , chemistry , heme , macromolecular crowding , lysozyme , ficoll , histidine , hemeprotein , biophysics , macromolecule , dissociation (chemistry) , biochemistry , stereochemistry , organic chemistry , enzyme , peripheral blood mononuclear cell , in vitro , biology
Myoglobin (Mb) undergoes pronounced heme loss under denaturing conditions wherein the proximal histidine gets protonated. Our data show that macromolecular crowding agents (both synthetic and protein based) can appreciably influence the extent of heme retention in Mb. Interestingly, glucose and sucrose, the monomeric constituents of dextran and ficoll‐based crowders were much more effective in preventing heme dissociation of Mb, albeit, at much higher concentrations. The protein crowders BSA and lysozyme show very interesting results with BSA bringing about the maximum heme retention amongst all the crowding agents used while lysozyme induced heme dissociation even in the native state of Mb. The stark difference that these protein crowders exhibit when interacting with the heme protein is a testament to the varied interaction potentials that a test protein might be exposed to in the physiological (crowded) milieu.